LAWSON (Luoshan China) company has developed a new type of tissue grinding in accordance with the Ministry of Environmental Protection, the standard for chlorophyll a "Determination of Chlorophyll a in Water Quality Spectrophotometry" HJ 897-2017.

Application of DHFSTPRP-Ch24  Sample Cell Freeze Grinder to Extract Chlorophyll Standard Method:
1. Filtration: add magnesium carbonate suspension before suction filtration to prevent pigment decomposition. The negative pressure cannot exceed 50kPa.
2. Extraction of chlorophyll: place the filter membrane in a DHFSTPRP-Ch24 extractor, add 10 ml of 90% acetone solution, and grind at 1500-2000 rpm for 1 min. Disrupt phytoplankton algal cells on the filter membrane. After grinding, soak in the dark at 4°C for extraction, and the soaking time is 15min to 24h. Final centrifugation for clarification: centrifuge at 1000 g for 20 min or filter and transfer the supernatant to a stopper glass vial.
3. Determination method: Spectrophotometry, three-color equation to measure the absorbance at 664nm, 647nm, 630nm, 750nm to calculate the chlorophyll content of chlorophyll a, b and c. Determination of pheophytin a corrected content by monochromatic equation
DHFSTPRP-Ch24 can process 24×15 mL centrifuge tubes at the same time for rapid cell disruption, cell lysis, and tissue homogenization.

Grinding samples include plant roots, stems, leaves, flowers, fruits, seeds and certain animal tissues; it is especially suitable for the extraction of nucleic acids, proteins and other components in plant tissues. In addition, it can also crush yeast, cultured animal cells, bacterial cells, etc., so as to extract the components therein.
The three-dimensional integrated oscillation mode makes the sample grinding more uniform and sufficient, with good sample repeatability and no cross-contamination between samples. It is a real high-throughput grinding and oscillation system. Each sample only takes 1-2 minutes to complete.
For temperature-sensitive samples, Ningbo Luoshan accessories can be used for cryogenic grinding and preservation.
DHFSTPRP-Ch24 Applicable samples and applications:
1. PCR (polymerase chain reaction) rapid multi-sample grinding preparation

2. Rapidly lyse tissue homogenate for nucleic acid extraction, RNA/DNA extraction, and protein extraction

3. Lysis of yeast and bacterial cells

4. Pesticide/insecticide analysis (in LC/MS/MS testing food residues pesticides or toxic substances sample processing

5. QuEChERS method (pesticide, antibiotic, drug extraction)

6. Extraction of medicinal active ingredients APIs

7. Food safety (contamination, adulteration, food traceability)

8. Biofuels

Experimental steps:

Key Features:

? Specially designed grinder for the extraction of nucleic acid, protein and its analytical components in plant samples

? High-throughput: It can grind 24*15ml samples at the same time, and it is suitable for grinding samples of various sizes, which greatly improves the work efficiency

? Each sample takes only 1-2 minutes to complete

? In order to ensure the reproducibility, validity and comparability of the samples, the grinding machine can set the grinding time, grinding rate and cycle times to ensure the consistency of each grinding and the next grinding conditions, the maximum time is 99 minutes.

? Built-in two safety locks and a sample compartment cover interlock, extremely safe and reliable.

? Expert in normal and low temperature animal and plant grinding

? Start and stop buttons allow the user to start or stop the grinding process at any time.

? Optional Ningbo Luoshan cryogenic accessories for cryogenic grinding and preservation of temperature-sensitive RNA and protein extraction samples

Technical parameters:

◆ Application areas: tissue homogenization, grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, shaking

◆ 24 samples can be processed simultaneously in 15 seconds, including 12-bit and 24-bit liquid nitrogen freezing adapters

◆ Compatible sample size: 24*(0.2-0.5ML) /24*2ML /8*(5-15)ML /4*25ML /2 *50ML, various specifications of grinding tubes can be customized at will

◆ Touch screen display for convenient and intuitive operation

◆ It can store ten sets of experimental data. According to different experimental samples, animal heart, spleen, lung, kidney, bone, skin, and hair patterns can be set.

◆ Mode cycle: According to the set experimental parameters, it can continuously cycle between several set parameters to further reduce the interference of human factors strong>

◆ Anti-vibration principle: DHFSTPRP-1 innovative anti-vibration principle, as well as a special three-dimensional vibration mode of up and down and left and right shaking, the sample moves in a three-dimensional figure 8 shape in space, innovative The unique grinding bead movement mode ensures ideal sample processing and instant pulverization effect

◆ Feed size: no requirement, adjust according to adapter

◆ Discharge particle size: ~5μm

◆ Number of grinding platforms (acceptable number of grinding jars) >2

◆ Fastening with automatic centering Yes

◆ Homogenization speed: 0—70 HZ/second, working time: 0 seconds-9999 minutes, user can set by themselves;

◆ Grinding Ball Diameter: 0.1-30mm

◆ Grinding Ball Material: Alloy Steel, Chrome Steel, Zirconia, Tungsten Carbide, Quartz Sand;

◆ Acceleration: Maximum speed in 2 seconds

◆ Slowdown: Reach lower speed in 2 seconds

◆ Noise Level: <55db

◆ Grinding Method: Wet Grinding, Dry Grinding, Low Temperature Grinding

◆ Ability to upgrade to cryogenic liquid nitrogen refrigeration or air cooler refrigeration

◆ Adapter Material: Teflon or Alloy Steel

◆ Fastening device with automatic centering, safety lock when working, full protection

◆ Grinding Kit Material Hard Rigid, Teflon (Teflon) Zirconia

◆ Dimensions: 450*500*580mm

◆ Weight: 55KG

Experiment results: